|
| Status |
Public on Mar 13, 2020 |
| Title |
Volunteer 01 |
| Sample type |
RNA |
| |
|
| Source name |
whole blood from healthy volunteer
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 24
disease state: healthy volunteer
tissue: whole blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA according to the standard protocol of WT Terminal Labeling and Controls Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 (Affymetrix).
|
| Description |
human_whole blood _11
|
| Data processing |
Affymetrix GeneChip Command Console (version 4.0, Affymetrix) software was used to extract raw data. Next, Expression Console (version1.3.1, Affymetrix) software offered RMA normalization for both gene and exon level analysis.
|
| |
|
| Submission date |
Mar 12, 2020 |
| Last update date |
Mar 13, 2020 |
| Contact name |
Jiayang Wu |
| E-mail(s) |
[email protected]
|
| Organization name |
China Pharmaceutical University
|
| Lab |
State Key Laboratory of Natural Medicines
|
| Street address |
No. 24 Tongjia Lane, Gulou District
|
| City |
Nanjing |
| ZIP/Postal code |
210009 |
| Country |
China |
| |
|
| Platform ID |
GPL23178 |
| Series (1) |
| GSE146882 |
Altered lncRNAs transcriptomic profiles in atherosclerosis-induced ischemic stroke |
|
