|
| Status |
Public on Aug 18, 2009 |
| Title |
SPS1 dsRNA treated cell at day 3, technical rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila SL2 cell of 3day after SPS1 dsRNA treatment
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: SL2
|
| Treatment protocol |
0.25×106 cells were plated on a 24-well plate in 0.5ml of HyQ SFX-Insect media. 4 mg of dsRNAs were added directly to the cells and incubated for 24 hrs and split into appropriate culture dishes for further incubation
|
| Growth protocol |
Drosophila Schneider cell line 2 (SL2) was cultured at 25℃ using HyQ SFX-Insect media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. The RNA quality was checked with Experion (Applied Biosystems) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 mg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45℃ on Gene Chip Drosophila Genome 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from SPS1 dsRNA treated cell at day3 (technical replication 2)
|
| Data processing |
The data were analyzed with Acuty 4.0 (Axon instrument) using a RMA (Robuster Multichip Analysis) as following 3step;background correction, normalization between chip using quantile normalization method, summerization using median polish method.
|
| |
|
| Submission date |
Aug 17, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Byeong Jae Lee |
| E-mail(s) |
[email protected]
|
| Phone |
+82-2-880-6775
|
| Fax |
+82-2-872-9019
|
| URL |
http://lmgg.snu.ac.kr
|
| Organization name |
Seoul National University
|
| Department |
School of Biological Sciences
|
| Lab |
Lab. of Molecular Genetics and Genomics
|
| Street address |
599 Gwanak-ro, Gwanak-gu,
|
| City |
Seoul |
| ZIP/Postal code |
151-742 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE17685 |
Gene expression profile in selenophosphate synthetase 1 (SPS1) knockdown Drosophila SL2 cell |
|
| Relations |
| Reanalyzed by |
GSE119084 |
