Strain: C57BL6 Placenta - E18, Female total RNA (MoPl.FE18) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples. Cells/tissue: Library MoPl.FE18.sig21 Cell type Placenta - E18, Female Source NHGRI RNA isolation LYNX mRNA QC passed cDNA library: Library DpnII restriction - (signature cloning using MmeI) Sequence length 20 bp MPSS: runs MoPl.FE18_sig21.5208F.a-20 10/7/2004 593548 11333 QC Passed MoPl.FE18_sig21.5208F.b-20 10/5/2004 600437 12576 QC Passed MoPl.FE18_sig21.5208W.c-20 11/19/2004 672002 14842 QC Passed MoPl.FE18_sig21.5322W.b-20 1/31/2005 484199 9584 QC Passed MoPl.FE18_sig21.5322W.d-20 2/18/2005 556369 11548 QC Passed Run group: Total Beads successfully sequenced - 2906555 Processed Signatures - 20318
