|
| Status |
Public on Jan 05, 2010 |
| Title |
Normal_4 |
| Sample type |
RNA |
| |
|
| Source name |
Homo Sapiens
|
| Organism |
Homo sapiens |
| Characteristics |
subtype: normal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
10 ug of Qiagen-purified total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Description |
Gene expression ratios were obtained from human Clear Cell kidney tumor and normal kidney tissues.
|
| Data processing |
The data were analyzed with custom CDF environments created by the MBNI - The version 11 entrez gene ID CDF was used.
|
| |
|
| Submission date |
Aug 26, 2009 |
| Last update date |
Aug 31, 2010 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL9101 |
| Series (2) |
| GSE17818 |
Somatic Mutation Screen of Clear Cell RCC II |
| GSE17895 |
Somatic Mutation Screen of Clear Cell RCC |
|
| Relations |
| Reanalyzed by |
GSM556068 |
