|
| Status |
Public on Apr 09, 2020 |
| Title |
WT_H1 |
| Sample type |
SRA |
| |
|
| Source name |
Cell pellet
|
| Organism |
Nakaseomyces glabratus |
| Characteristics |
strain: KUE100
genotype: WT
treatment: hydrogen peroxide
|
| Treatment protocol |
Cell pellets were collected and immediately stored at -80ºC
|
| Growth protocol |
Cell were grown in minimal medium until early exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion Ribopure-Yeast RNA kit, according to manufacturer’s instructions.
Illumina Truseq Stranded Total RNA library prep kit with ribosomal depletion via RiboZero Gold according to the manufacturer’s protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Quality control of reads was assessed with FastQC
Trimming of the reads was performed with Skewer
Mapping to the reference genome was done using TopHat
Counts per gene were calculated with HTSeq
Differential gene expression analysis was calculated with DESeq2
Genome_build: CBS138
Supplementary_files_format_and_content: Counts files containing the genome's ORFs and the number of read counts for each ORF
|
| |
|
| Submission date |
Apr 08, 2020 |
| Last update date |
Jan 19, 2022 |
| Contact name |
Miguel Cacho Teixeira |
| Organization name |
Instituto Superior Tecnico
|
| Department |
Institute for Bioengineering and Biosciences
|
| Street address |
Avenida Rovisco Pais 1
|
| City |
Lisbon |
| ZIP/Postal code |
1049-001 |
| Country |
Portugal |
| |
|
| Platform ID |
GPL26394 |
| Series (1) |
| GSE148288 |
A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: the transcription factor CgTog1 |
|
| Relations |
| BioSample |
SAMN14558564 |
| SRA |
SRX8081984 |
