|
| Status |
Public on Mar 31, 2010 |
| Title |
GFP dsRNA treated control 1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila S2 cells treated with GFP dsRNA (Day 5)
|
| Organism |
Drosophila melanogaster |
| Characteristics |
agent: GFP dsRNA
tissue: S2 tissue culture cells
|
| Treatment protocol |
Cells were grown in 25 cm2 flasks, treated with dsRNAs (30µg/ml) on days 0, 1, and 3, resuspended by pipetting each time dsRNA was added, and lysed using TRIzol on day 5.
|
| Growth protocol |
S2 Cells were grown in HyQ CCM3 media at 27 degrees
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized using Genechip Drosophila_2 microarrays (Affymetrix), washed, and data acquired via GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from S2 cells treated with indicated dsRNA
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and default normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Aug 28, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Daniel Louis Kiss |
| E-mail(s) |
[email protected]
|
| Phone |
216-368-0385
|
| Fax |
216-368-3055
|
| Organization name |
Case Western Reserve University
|
| Department |
Molceular Biology and Microbiology
|
| Lab |
Andrulis
|
| Street address |
2109 Adelbert Road
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE17874 |
Expression data from Drosophila S2 cells depleted of exosome subunits |
|
| Relations |
| Reanalyzed by |
GSE119084 |
