Liver was obtained from 8 or 9 male, two-month-old, mice for each transgenic model. Three different models were compared, including liver-Cpr-null, Cpr-low, and Cpr-lox (control model), all on mixed C57BL/6 and 129/Sv genetic background. Total RNA was prepared from individual liver samples. Three independent pools of RNA samples were prepared and analyzed for each model. Five micrograms of total RNA were used for the synthesis of biotin-labeled antisense RNA using Ambion's MESSAGEAMP(TM) aRNA Kit. Keywords = Cytochrome P450 Keywords = NADPH-cytochrome P450 reductase Keywords = transgenic mice Keywords = liver Keywords = nuclear recetpor
