|
| Status |
Public on Nov 01, 2009 |
| Title |
Fibroblast 3 |
| Sample type |
RNA |
| |
|
| Source name |
fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Detroit 551 fetal skin fibroblast from ATCC
|
| Growth protocol |
Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. iPS cells were cultured in hES medium (80% DMEM/F12, 20% KO Serum Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
|
| Label |
biotin
|
| Label protocol |
Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
|
| |
|
| Hybridization protocol |
cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
|
| Scan protocol |
Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
|
| Description |
Gene expression data obtained from fibroblasts
|
| Data processing |
RMA
|
| |
|
| Submission date |
Sep 15, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Akiko Doi |
| E-mail(s) |
[email protected]
|
| Phone |
410-614-3478
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Medicine
|
| Street address |
855 N Wolfe St, Rangos 580
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE18111 |
Human iPS cells and fibroblasts |
| GSE18226 |
Expression data from human iPS cells and fibroblasts |
|
| Relations |
| Reanalyzed by |
GSE119087 |
