tissue: head exposure type: Ethanol minutes after exposure: 90 gender: male
Treatment protocol
Flies were either untreated (2 replicates), or treated with 30 min ethanol vapor or humidified air. RNA was extracted 0, 30, 60, 90, 120, 180, or 210 min following exposure termination, and were snap frozen in liquid nitrogen.
Growth protocol
200 2-4 day old male flies from synchronized cultures were used for all conditions
Extracted molecule
total RNA
Extraction protocol
RNA was extracted using the Trizol reagent
Label
biotin
Label protocol
single labeling protocol
Hybridization protocol
Affymetrix standard protocol
Scan protocol
Affymetrix standard protocol
Description
Head RNA extracted 90' after Drosophila exposed for 30' to ethanol.
