|
| Status |
Public on Nov 04, 2009 |
| Title |
MEF rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts (MEFs)
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Embryonic Fibroblast Cells
|
| Treatment protocol |
Cells were treated with small molecules as described in Ichida et al. (Cell Stem Cell, 2009).
|
| Growth protocol |
Cells were grown in a monolayer on gelatin-coated plates, either with or without an irradiated mouse embryonic fibroblast (MEF) feeder layer. They were given standard mES media containing either fetal bovine serum (FBS) or knock-out serum replacement (KSR).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent in accordance with the prescribed protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA Amp kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Non-normalized data file: RAW_2.txt
|
| Data processing |
The data were normalized using cubic-spline normalization with BeadStudio.
|
| |
|
| Submission date |
Sep 25, 2009 |
| Last update date |
Sep 25, 2009 |
| Contact name |
Esther Yesde Son |
| E-mail(s) |
[email protected]
|
| Phone |
617-496-5611
|
| Fax |
617-384-5611
|
| Organization name |
Harvard University
|
| Department |
Stem Cells and Regenerative Biology
|
| Lab |
Eggan
|
| Street address |
7 Divinity Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE18286 |
Global gene expression analysis of (1) chemically reprogrammed iPS cells, and (2) chemical-treated cells |
|
