|
Status |
Public on Oct 01, 2013 |
Title |
KO_cycling_rep3 |
Sample type |
RNA |
|
|
Source name |
KO MEF cycling cells
|
Organism |
Mus musculus |
Characteristics |
cell type: mouse embryonic fibroblasts (MEF) phase: cycling
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Ambion mirVana, in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol, with Illumina iScan System.
|
Description |
4655400089_B
|
Data processing |
The data were normalised using quantile normalisation with BeadStudio 3.0
|
|
|
Submission date |
Oct 07, 2009 |
Last update date |
Oct 01, 2013 |
Contact name |
Riccardo Lorenzo Rossi |
E-mail(s) |
[email protected]
|
URL |
http://www.ingm.it
|
Organization name |
Fondazione INGM
|
Department |
Integrative Biology
|
Lab |
Pagani
|
Street address |
Via Trivulzio 10
|
City |
Milano |
State/province |
MI |
ZIP/Postal code |
20149 |
Country |
Italy |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE18463 |
The effects of Hmgb1 depletion on gene expression in murine cells |
|