|
| Status |
Public on Oct 01, 2013 |
| Title |
KO_cycling_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
KO MEF cycling cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblasts (MEF)
phase: cycling
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Ambion mirVana, in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol, with Illumina iScan System.
|
| Description |
4655400089_D
|
| Data processing |
The data were normalised using quantile normalisation with BeadStudio 3.0
|
| |
|
| Submission date |
Oct 07, 2009 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Riccardo Lorenzo Rossi |
| E-mail(s) |
[email protected]
|
| URL |
http://www.ingm.it
|
| Organization name |
Fondazione INGM
|
| Department |
Integrative Biology
|
| Lab |
Pagani
|
| Street address |
Via Trivulzio 10
|
| City |
Milano |
| State/province |
MI |
| ZIP/Postal code |
20149 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE18463 |
The effects of Hmgb1 depletion on gene expression in murine cells |
|
