|
| Status |
Public on Jun 11, 2020 |
| Title |
Replicate 4 Blood_Fed_day 3 |
| Sample type |
SRA |
| |
|
| Source name |
Ovary
|
| Organism |
Aedes aegypti |
| Characteristics |
age: 4-7 day old mosquito
diet: Fed with blood meal
timepoint: Ovary collected on day 3 PBM
|
| Treatment protocol |
All mosquitoes, both sugar fed and blood fed, were maintained with 10% sucrose and reared identically. Ovary samples were collected on day 3, 10, and 20 post blood meal (PBM) and stored immediately at -80oC.
|
| Growth protocol |
4-7 days old A. aegypti (from Poza Rica, Mexico) were fed with warmed sheep blood. No additional blood meals were given for the entire study period. Control mosquitoes were given sugar meal.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the mirVana miRNA isolation kit (Invitrogen) following the manufacturer’s suggestions.
RNA libraries were prepared following the Illumina Stranded total RNA library prep kit with Ribo-Zero Gold protocol.
Following library concentration and quality control, final libraries were pooled to 10 nM. After dilution and denaturing, the pooled library was loaded onto a NovaSeq6000 S1 flow cell (PE200) for sequencing.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
RNA was isolated using mrVana miRNA kisolation kit
|
| Data processing |
The raw sequences were subjected to quality check by FastQC
Bioinformatics analysis was carried out using CLC Genomics workbench v.20 software.
Gene expression tracks after mapping are shown
Genome_build: Aedes-aegypti-LVP_AGWG_CHROMOSOMES_AaegL5.fa.gz
|
| |
|
| Submission date |
Jun 10, 2020 |
| Last update date |
Jun 11, 2020 |
| Contact name |
Dilip K Nag |
| E-mail(s) |
[email protected]
|
| Organization name |
Griffin Laboratory, Wadsworth Center, New York State Department of Health
|
| Department |
Division of Infectious Diseases
|
| Lab |
Arbovirus Laboratory
|
| Street address |
5668 State Farm Road
|
| City |
Slingerlands |
| State/province |
NY |
| ZIP/Postal code |
12159 |
| Country |
USA |
| |
|
| Platform ID |
GPL28657 |
| Series (1) |
| GSE152209 |
RNAseq analysis of blood-meal induced gene-expression changes in Aedes aegypti ovaries |
|
| Relations |
| BioSample |
SAMN15198719 |
| SRA |
SRX8521025 |
