|
| Status |
Public on Jun 18, 2021 |
| Title |
99PF Liver Donor |
| Sample type |
RNA |
| |
|
| Source name |
Liver tissue
|
| Organism |
Homo sapiens |
| Characteristics |
age stage: Old (>40 years)
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 50 ng total RNA (GeneChip Expression Analysis Technical Manual, 2005-2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 to 18 µg (mean 12.8 µg) of cRNA was hybridized for 16 hr at 45°C on Affymetrics Human U133 Plus 2 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner 3000_7Gplus
|
| Data processing |
Affymetrics probeset summaries were computed using MAS 5.0 algorithm. Data were then imported to BRB-ArrayTools and subsequent data filtering, median normalization and statistical analyses were performed using this software.
|
| |
|
| Submission date |
Jun 18, 2020 |
| Last update date |
Jun 18, 2021 |
| Contact name |
Ronald E Engle |
| E-mail(s) |
[email protected]
|
| Phone |
240-669-5927
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
LID HPS
|
| Street address |
50 South Drive 6345
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE152738 |
Chitinase-3 Like 1 Is a Profibrogenic Factor Overexpressed in Aging Livers and in Patients with Cirrhosis in the Aging Liver and in Patients with Liver Cirrhosis |
|
