|
| Status |
Public on Jan 19, 2010 |
| Title |
A435_2_LPS |
| Sample type |
RNA |
| |
|
| Source name |
A435_2_LPS
|
| Organism |
Mus musculus |
| Characteristics |
agent: LPS
|
| Treatment protocol |
BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
|
| Growth protocol |
BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
|
| |
|
| Hybridization protocol |
The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
|
| Description |
Gene expression data from BV-2 cells treated with 50 ng/ml LPS
|
| Data processing |
RMA
|
| |
|
| Submission date |
Oct 26, 2009 |
| Last update date |
Mar 29, 2010 |
| Contact name |
Thomas Langmann |
| E-mail(s) |
[email protected]
|
| Phone |
+49-941-944-5423
|
| Fax |
+49-941-944-5402
|
| Organization name |
Institute of Human Genetics
|
| Department |
University of Regensburg
|
| Street address |
Franz-Josef-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18740 |
Luteolin has anti-inflammatory and neurotrophic effects on microglia |
|
