|
| Status |
Public on Oct 28, 2009 |
| Title |
wild-type DT40 cell |
| Sample type |
RNA |
| |
|
| Source name |
Gallus gallus DT40 B cell wild-type clone
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: bursa of Fabricius
cell line: DT40 B cell line
genotype/variation: wildtype
|
| Growth protocol |
Both wild-type and PTIP deficient DT40 cells were cultured at 39°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNAs were isolated from wild-type and PTIP-/-/- cells using Sepasol®-RNA I (Nacalai tesque, Japan).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using GeneChip® Expression 3’ Amplification One-Cycle Target Labeling and Control Reagents (Affymetrix Cat#900493)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Genechip® Chicken Genome Array (Affymetrix Cat#900590). GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix P/N00-0079).
|
| Scan protocol |
GeneChips were scanned using GeneChip® Scanner 3000 7G System w/Workstation and AutoLoader (Affymetrix P/N00-0218)
|
| Description |
no additional information
|
| Data processing |
The data were analyzed with GeneChip® Operating Software (GCOS) (Affymetrix P/N690-036)
|
| |
|
| Submission date |
Oct 28, 2009 |
| Last update date |
Oct 28, 2009 |
| Contact name |
XIN WANG |
| E-mail(s) |
[email protected]
|
| Phone |
81-75-7534410
|
| Fax |
81-75-7534419
|
| Organization name |
Kyoto University
|
| Department |
Graduate School of Medicine
|
| Lab |
Radiation Genetics
|
| Street address |
Yoshida Konoe, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE18778 |
Comparison of gene expression between wild-type and PTIP deficient chicken DT40 B cells |
|
