|
| Status |
Public on Aug 07, 2010 |
| Title |
T6PNE cells, E47 induced (1273001003_C) |
| Sample type |
RNA |
| |
|
| Source name |
T6PNE cells were isolated from human fetal islets; E47 was induced by tamoxifen treatment
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreas islets
cell line: fetal islet cell line T6PNE
protocol: E47 induction
|
| Treatment protocol |
E47 was induced in T6PNE with Tamoxifen (0.5-4µM). Islets were untreated.
|
| Growth protocol |
TRM 6 cells were derived from human fetal islets. They were engineered to express PDX-1, NeuroD1 and inducible E45. Both islets and T6PNE RPMI media with 5.5mM glucose 10% FBS and 1% pen/strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested with RLT buffer and βME. RNA was extracted with DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
streptavidin Cy3
|
| Label protocol |
As suggested by illumina
|
| |
|
| Hybridization protocol |
As recommended by illumina
|
| Scan protocol |
As suggested by illumina using the illumina scanner
|
| Description |
no additional information
|
| Data processing |
Raw data filtered on P value for <0.05 with the remaining data normalised using the affy tools R bioconductor normalize.quantiles routine
VALUE = Quantile-normalized signal
|
| |
|
| Submission date |
Oct 30, 2009 |
| Last update date |
Mar 29, 2010 |
| Contact name |
Roy M Williams |
| E-mail(s) |
[email protected]
|
| Organization name |
The Scripps Research Institute
|
| Department |
Regenerative Medicine
|
| Lab |
Loring
|
| Street address |
3030 Science Park Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL2725 |
| Series (1) |
| GSE18821 |
Phenothiazine Neuroleptics Signal To The Human Insulin Promoter As Revealed By A Novel Human b-Cell Line Based Screen |
|
