|
| Status |
Public on Jul 08, 2021 |
| Title |
ZWT105 KO CAR 3 |
| Sample type |
RNA |
| |
|
| Source name |
transferred CAR T cells sorted from spleens of B6 recipients
|
| Organism |
Mus musculus |
| Characteristics |
strain: CAR transgenic mice were bred on a B6 background and then to Thy1.1 and Rosa26-Cas9 knockin mice.
genotype/variation: KO CAR T cells (Ametrine+Thy1.1–Thy1.2+)
|
| Treatment protocol |
Sorted cells were lysed with lysis buffer from RNeasy Micro kit from Qiagen.
|
| Growth protocol |
CD8+ CAR T cells were isolated by flow cytometric sorting of splenocytes based on reporters and congenical markers: WT CAR (mCherry+Thy1.1+Thy1.2+) and KO CAR T cells (Ametrine+Thy1.1–Thy1.2+) 21 days after co-transfer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of RNeasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Jul 22, 2020 |
| Last update date |
Jul 09, 2021 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
[email protected]
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE154942 |
Regnase-1 KO CAR T cell reprogramed to memory-like cells |
| GSE155021 |
Regnase-1 suppresses TCF-1+ precursor exhausted T cell formation to limit CAR T cell responses against ALL |
|
