|
| Status |
Public on Jul 30, 2011 |
| Title |
MCF10A EGF treated 3days |
| Sample type |
RNA |
| |
|
| Source name |
MCF10A EGF treated 3days
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
genome/variation: wild type
treatment group: EGF treated
length of treatment (days): 3
|
| Treatment protocol |
Cells were grown in the absence or presence of EGF.
|
| Growth protocol |
Trypsin-treated cells were re-suspended in DME/F12 medium supplemented with 2% horse serum, insulin, cholera toxin and hydrocortisone. Eight-chambered plates (BD Biosciences) were coated with 35 µl MatrigelTM per well. The cells were mixed 1:1 with assay medium containing MatrigelTM (4%) and EGF (20 ng/ml), and 400 µl added to each chamber.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and RNA isolated using a Versagene kit (Gentra Systems, Minneapolis).
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix instructions
|
| |
|
| Hybridization protocol |
According to Affymetrix instructions
|
| Scan protocol |
According to Affymetrix instructions
|
| Description |
A527_C_Eplus_3D
|
| Data processing |
Data was normalized by RMA method using Command Console CHP file version 1.
|
| |
|
| Submission date |
Nov 09, 2009 |
| Last update date |
Jul 30, 2011 |
| Contact name |
Amit Zeisel |
| E-mail(s) |
[email protected]
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Physics of complex systems
|
| Lab |
Prof. Eytan Domany
|
| Street address |
Weizmann Institute
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE18938 |
Effect of EGF and/or HER2 on the growth of MCF10A cells on extracellular matrix: time course |
|
