|
| Status |
Public on Dec 10, 2009 |
| Title |
Liver-0mg-R4 |
| Sample type |
RNA |
| |
|
| Source name |
Liver-0mg-R4
|
| Organism |
Colinus virginianus |
| Characteristics |
dose: 0 mg 2,6 DNT
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was fixed in RNA Later. RNA was extracted using Qiagen, Mini-prep extraction columns. RNA quality was assessed by NanoDrop analysis followed by Agilent, Bioanalyzer, NanoChip electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Agilent One-Color hybridization kits and protocol were used. One microgram of RNA was used / biological replicate as starting material.
|
| |
|
| Hybridization protocol |
We followed the Agilent One-Color hybridization protocal for all aspects of the hybridization
|
| Scan protocol |
An Axon GenePix® 4000B Microarray Scanner (MDS Analytical Technologies Inc., Toronto, Canada) was used to scan microarrays at 5 μm resolution.
|
| Description |
n/a
|
| Data processing |
Data were extracted from microarray images using Agilent Feature Extraction software (Agilent Technologies). Background subtracted adjusted median signal intensities were normalized on a per-chip basis that transforms the signal intensity by dividing signal intensity for each gene, with the mean intensity in each array.
|
| |
|
| Submission date |
Nov 09, 2009 |
| Last update date |
Nov 17, 2009 |
| Contact name |
Arun Rawat |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Southern Mississippi
|
| Department |
Biological Sciences
|
| Street address |
118 College Drive # 5018
|
| City |
Hattiesburg |
| State/province |
MS |
| ZIP/Postal code |
39406 |
| Country |
USA |
| |
|
| Platform ID |
GPL9548 |
| Series (1) |
|
