|
| Status |
Public on Nov 25, 2022 |
| Title |
AbSeq tag oligo library |
| Sample type |
SRA |
| |
|
| Source name |
Ab tag oligo
|
| Organism |
synthetic construct |
| Characteristics |
disease: NA
molecule: AbSeq tag
|
| Extracted molecule |
other |
| Extraction protocol |
isolated Δ42PD-1+, PD-1+ T and Δ42PD-1-PD-1- T cells were labeled with sample tag and Ab tag before loaded into BD Phapsody for RNA exraction, RNA was extracted according to BD Rhapsody protocol
library was prepared according to BD Rhapsody protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Average high-quality (> Q30) base calls of 84%
Sequenced reads were trimmed to 75 bp
Raw reads were process with BD Rhapsody SBG cloud pipeline
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every T cell subset
Supplementary_files_format_and_content: sample tag metrics: HC1_Sample_Tag_Metrics.csv
Supplementary_files_format_and_content: Δ42PD-1-PD-1- T cell subsets: HC1_SampleTag01_hs_neg_DBEC_MolsPerCell.csv
Supplementary_files_format_and_content: Δ42PD-1+ T cell subsets: HC1_SampleTag02_hs_d42_DBEC_MolsPerCell.csv
Supplementary_files_format_and_content: PD-1+ T cell subsets: HC1_SampleTag03_hs_pd1_DBEC_MolsPerCell.csv
|
| |
|
| Submission date |
Aug 05, 2020 |
| Last update date |
Nov 25, 2022 |
| Contact name |
Zhiwu Tan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Hong Kong
|
| Department |
Microbiology
|
| Lab |
AIDS Institute
|
| Street address |
21 Sassoon Road, Pokfulam
|
| City |
Hong Kong |
| ZIP/Postal code |
000000 |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL17769 |
| Series (1) |
| GSE155736 |
Isoformic PD-1 is tumorigenic in hepatocellular carcinoma through seemingly exhausted αβ T cells |
|
| Relations |
| BioSample |
SAMN15735170 |
| SRA |
SRX8890302 |
