|
| Status |
Public on Jan 24, 2021 |
| Title |
RM6/66-3 |
| Sample type |
SRA |
| |
|
| Source name |
Gram-negative bacteria
|
| Organism |
Brucella canis |
| Characteristics |
strain: RM6/66 (parental)
|
| Treatment protocol |
Brucella canis RM6/66 was obatined from IVDC, and the ΔmucR strains was constructed from its parent strain RM6/66
|
| Growth protocol |
Brucella canis RM6/66 and ΔmucR strains were grown in TSB at 37℃ until the log phase was reached
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen).
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: ASM74033v1
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Aug 05, 2020 |
| Last update date |
Jan 24, 2021 |
| Contact name |
Hao Dong |
| E-mail(s) |
[email protected]
|
| Phone |
+86-10-53852705
|
| Organization name |
National Institutes for Food and Drug Control
|
| Street address |
Huatuo road NO.31
|
| City |
Beijing |
| ZIP/Postal code |
102618 |
| Country |
China |
| |
|
| Platform ID |
GPL28970 |
| Series (1) |
| GSE155748 |
Comparative transcriptome analysis of Brucella canis MucR mutant strain and its parent strain RM6/66 |
|
| Relations |
| BioSample |
SAMN15738053 |
| SRA |
SRX8893889 |
