|
| Status |
Public on Sep 02, 2020 |
| Title |
Control |
| Sample type |
RNA |
| |
|
| Source name |
Human Gingival Epithelial Cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Gingiva
cell type: Human Gingival Epithelial Cell
treatment: control
|
| Treatment protocol |
The cells were treated with 50 μg/ml arecoline hydrobromide for 3 days and without arecoline for another 3 days for a total period of 1 month
|
| Growth protocol |
Human gingival epithelial progenitors were cultured in CnT-Prime epithelial cell culture medium containing antibiotics (5% penicillin-streptomycin at 37˚C in an incubator supplied with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the acid guanidine thiocyanate/phenol-chloroform method using TRIzol Reagent (Invitrogen Corporation, USA)
|
| Label |
Agilent Low Input QuickAmp Labeling Kit
|
| Label protocol |
50 ng of total RNA was was labelled using Agilent low input QuickAmp, one color
|
| |
|
| Hybridization protocol |
According to Agilent protocol
|
| Scan protocol |
According to Agilent protocol
|
| Data processing |
Feature extraction software from Agilent using default parameters (protocol GE1_107_Sep09 and Grid: 039494_D_F_20120628 012391_D_20060331)
Normalization was performed using software R. The quantile method was used for normalization.
|
| |
|
| Submission date |
Sep 01, 2020 |
| Last update date |
Sep 02, 2020 |
| Contact name |
Durga Paudel |
| E-mail(s) |
[email protected]
|
| Organization name |
Health Sciences University of Hokkaido
|
| Street address |
Kanazawa
|
| City |
Ishikari-Tobetsu |
| State/province |
Hokkaido |
| ZIP/Postal code |
061-0293 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE157247 |
Gene expression analysis of arecoline treated human gingival epithelial cells |
| GSE157248 |
Methylation and gene expression microarray of human gingival epithelial cells stimulated with arecoline |
|
