|
| Status |
Public on Feb 01, 2010 |
| Title |
Failing (rep 5) |
| Sample type |
RNA |
| |
|
| Source name |
Failing (rep 5)
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue type: heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from six biological replicates corresponding to Failing, Control, Failing+Captopril and Failing+Captopril+butyrate heart tissue using Trizol (Invitrogen) according to the manufacturer’s protocol, and the RNeasy mini-kit (Qiagen) was used to clean-up the RNA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from cell line
|
| Data processing |
data were processed with RMA method
|
| |
|
| Submission date |
Nov 26, 2009 |
| Last update date |
Dec 08, 2009 |
| Contact name |
steve shen |
| Organization name |
New York University
|
| Department |
Biochemistry
|
| Street address |
545 First Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE19210 |
Transition from Compensated Hypertrophy to Systolic Heart Failure in the Spontaneously Hypertensive Rat |
|
