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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 15, 2021 |
| Title |
WT tumor 5 |
| Sample type |
SRA |
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| Source name |
mammary gland tumor
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| Organism |
Mus musculus |
| Characteristics |
strain: FVB/n
disease state: tumor
genotype/variation: WT
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| Extracted molecule |
total RNA |
| Extraction protocol |
1 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra RNA Library Prep Kit for Illumina® (cat# E7420S, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
total RNA isolated from mammary gland tumors developing in control GAL4 mice
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| Data processing |
The RNA sequences were generated on an Illumina Nova 6000 Platform using a paired-end 2×150 bases.
RNA sequences were aligned against mouse genome mm10 by STAR (Version 2.7.3), and RNA expression was calculated by RSEM (RNA-Seq by Expectation Maximization).
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
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| Submission date |
Sep 17, 2020 |
| Last update date |
Sep 15, 2021 |
| Contact name |
Howard Yang |
| E-mail(s) |
[email protected]
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| Phone |
2402765257
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| Organization name |
NCI
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| Street address |
9609 Medical Center Dr.
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| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE158134 |
Progesterone promotes immunomodulation and tumor development in the murine mammary gland |
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| Relations |
| BioSample |
SAMN16197971 |
| SRA |
SRX9141130 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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