|
| Status |
Public on Sep 17, 2020 |
| Title |
early_sham_L_49_49L_a3_hypo |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
early_sham_skin_hypo
|
| Organism |
Sus scrofa domesticus |
| Characteristics |
breed/model: red Duroc swine scar model
gender: Male
age: Juvenile
pigmentation phenotype: hypo
tissue: Skin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Fibrous Tissue Kit based on the manufacturer’s protocol (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Total RNA (200-2000ng) was used as starting material, which was amplified using the Quick Amp Two-Color Kit (Agilent Technologies).The labeled cDNA was purified using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and eluted, labelled cRNA was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc, Waltham, MA, USA).
|
| |
|
| Channel 2 |
| Source name |
Zyagen Universal Pig Reference Total RNA
|
| Organism |
Sus scrofa domesticus |
| Characteristics |
gender: Unknown
age: Unknown
sample type: Reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Fibrous Tissue Kit based on the manufacturer’s protocol (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Total RNA (200-2000ng) was used as starting material, which was amplified using the Quick Amp Two-Color Kit (Agilent Technologies).The labeled cDNA was purified using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and eluted, labelled cRNA was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc, Waltham, MA, USA).
|
| |
|
| |
| Hybridization protocol |
cRNA was hybridized to a pig genome microarray (Agilent Technologies) following manufacturer's instructions
|
| Scan protocol |
Scanned on a Agilent Technologies Scanner G2505C Images were quantified using Agilent Feature Extraction Software
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.1.1) was used for background subtraction and GeneSpring v.10.1 (Agilent Technologies, Inc., CA) software was used to conduct the preliminary data filtration and statistical analysis.
|
| |
|
| Submission date |
Sep 17, 2020 |
| Last update date |
Sep 17, 2020 |
| Contact name |
Aarti Gautam |
| E-mail(s) |
[email protected]
|
| Phone |
301-619-7683
|
| Organization name |
WRAIR
|
| Department |
Medical Readiness Systems Biology
|
| Street address |
503 Robert Grant Avenue
|
| City |
Silver Spring |
| State/province |
MD |
| ZIP/Postal code |
20910 |
| Country |
USA |
| |
|
| Platform ID |
GPL16571 |
| Series (1) |
| GSE158155 |
Dyspigmented hypertrophic scars: Beyond skin color |
|
