|
| Status |
Public on Mar 01, 2010 |
| Title |
Breast-BRCA2-1 |
| Sample type |
RNA |
| |
|
| Source name |
Breast BRCA2 'one-hit' mutant cells from subject 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Epithelial cell culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared from cultured cells by extraction in guanidinium isothiocyanate-based buffer containing -mercaptoethanol and acid phenol
|
| Label |
biotin
|
| Label protocol |
RNA was biotinylated
|
| |
|
| Hybridization protocol |
each sample a total of 2.2 ug of ssDNA, labeled and fragmented with the NuGEN kit, was hybridized to Affymetrix arrays
|
| Scan protocol |
arrays were scanned with the Affymetrix GeneChip Scanner 3000
|
| Description |
RNA from Breast BRCA2 mutant
|
| Data processing |
CEL fles were normalized using RMA and bioconductor
|
| |
|
| Submission date |
Dec 08, 2009 |
| Last update date |
Dec 08, 2009 |
| Contact name |
suraj peri |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Fox chase cancer center
|
| Department |
biostatistics and bioinformatics
|
| Street address |
333 cottman ave
|
| City |
philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19111 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19383 |
Altered gene expression in normal breast and ovarian epithelial cells from BRCA1 and BRCA2 mutation carriers |
|
