|
| Status |
Public on Nov 29, 2010 |
| Title |
EN04_T2DM_T52 |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle vastus lateralis
|
| Organism |
Homo sapiens |
| Characteristics |
group: T2DM T52
age: 56
bmi: 31.90
|
| Treatment protocol |
After biopsy, the muscle samples were immediately frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the TRIzol reagent and purified with the Rneasy clean-up kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
150ng muscle RNA was reverse transcribed into cDNA and amplified in a two-round amplification according to the manufacturer's protocol (Affymetrix)
|
| |
|
| Hybridization protocol |
A mixture of cDNA and added hybridization controls was hybridized on Affymetrix HG-U133 Plus 2.0 chips, followed by staining and washing steps in the Affymetrix GeneChip fluidics station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data of muscle from control subject C7
|
| Data processing |
The data were analyzed with R, a free software program for statistical computing and graphics. Normal linear regression models were used to asses significantly differentially expressed genes between patients and controls.
|
| |
|
| Submission date |
Dec 10, 2009 |
| Last update date |
Sep 19, 2010 |
| Contact name |
Florence van Tienen |
| E-mail(s) |
[email protected]
|
| Organization name |
Maastricht University
|
| Department |
Genetics and Cell biology
|
| Street address |
Universiteitssingel 50
|
| City |
Maastricht |
| ZIP/Postal code |
6229ER |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19420 |
Skeletal muscle mitochondrial dysfunction is secondary to T2DM |
|
