|
| Status |
Public on Oct 20, 2020 |
| Title |
C3: in utero oxycodone exposure |
| Sample type |
SRA |
| |
|
| Source name |
Prefrontal Cortex
|
| Organism |
Rattus norvegicus |
| Characteristics |
age: post-natal day 14
strain: Sprague Dawley Rat
tissue: Prefrontal Cortex
treatment: in utero oxycodone exposure
|
| Treatment protocol |
For the in utero exposure animals (IUO), an ascending dosing procedure was used wherein doses of 10 mg/kg/day oxycodone (oxy) were orally gavaged for 5 days followed by 0.5 mg/kg/day escalation for 10 days to a final dose of 15 mg/kg/day, after which F0 females were mated with proven male breeders, and the treatment regimen continued throughout mating, gestation, and parturition until weaning. For post-natal exposure (PNO), F0 dams were orally gavaged with 15 mg/kg/day of oxy after parturition until weaning.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The prefrontal cortex was removed from the brains of P14 rats. Tissues were frozen on dry ice. Total RNA was isolated from each sample using the Zymo Direct-Zol RNA kit according to manufacturer's instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
RNAseq libraries were generated by the UNMC NGS Core beginning with 1 ug of total RNA from each sample using the TruSeq V2 RNA sequencing library kit from Illumina following recommended procedures
Resultant libraries were assessed for size of insert by analysis of an aliquot of each library on a BioAnalyzer instrument
Multiplexed libraries (18 samples per pool) were sequenced on a single flow cell of the NextSeq550 DNA Analyzer (Illumina) to generate a total of approximately 28 million 75 bp single reads for each sample.
Genome_build: GCA_000001895.4
|
| |
|
| Submission date |
Oct 19, 2020 |
| Last update date |
Oct 20, 2020 |
| Contact name |
Sowmya V Yelamanchili |
| E-mail(s) |
[email protected]
|
| Phone |
8589266110
|
| Organization name |
UNIVERSITY OF NEBRASKA MEDICAL CENTER
|
| Department |
Anesthesiology
|
| Street address |
984455 Nebraska Medical Center
|
| City |
OMAHA |
| State/province |
Nebraska |
| ZIP/Postal code |
68198-4455 |
| Country |
USA |
| |
|
| Platform ID |
GPL25029 |
| Series (1) |
| GSE159563 |
A holistic systems approach to characterize the impact of pre- and post-natal oxycodone exposure on neurodevelopment and behavior |
|
| Relations |
| BioSample |
SAMN16474982 |
| SRA |
SRX9300829 |
