|
| Status |
Public on Aug 19, 2021 |
| Title |
control-36 |
| Sample type |
SRA |
| |
|
| Source name |
exosome from plasma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: exosome from plasma
age: 42 years old
disease state: healthy individual
|
| Extracted molecule |
total RNA |
| Extraction protocol |
2mL of venous blood from patients were collected into Ethylene Diamine Tetraacetic Acid (EDTA) routine blood tubes. Plasma was separated by centrifugation at 3000 rpm for 10 min at 4℃ and collected into cryogenic vials and stored at −80℃. The exoRNeasy Serum/Plasma kit (Qiagen) was applied to extract the exosomal total RNAs.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using bowtie hisat2 with parameters -p 12 -5 5 -3 5 --rna-strandness RF
HTseq was used to calculate the counts of the Reads mapped the genome.
Genome_build: hg38
Supplementary_files_format_and_content: Matrix table with Htseq-counts
|
| |
|
| Submission date |
Oct 20, 2020 |
| Last update date |
Oct 29, 2021 |
| Contact name |
guodong he |
| E-mail(s) |
[email protected]
|
| Phone |
8613660816883
|
| Organization name |
Guangdong Provincial People's Hospital
|
| Street address |
zhongshan road No.102
|
| City |
Guangzhou |
| ZIP/Postal code |
510100 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE159657 |
The alteration of circulating exosomal mRNAs in acute myocardial infarction is associated with inflammation response |
|
| Relations |
| Alternative to |
GSM5662278 |
| BioSample |
SAMN16483443 |
| SRA |
SRX9316490 |
