|
| Status |
Public on Jan 01, 2021 |
| Title |
endothelial cells, G1/S phase, rep 5 |
| Sample type |
SRA |
| |
|
| Source name |
endothelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hESC-derived endothelial cells
treatment: untreated
|
| Treatment protocol |
All cell were untreated
|
| Growth protocol |
Differentiation of cells were performed based on Qiu et al. Cell Reports. 2020.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All mRNA samples were isolated using RNeasy Plus Micro Kit (Qiagen, cat# 74034).
Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Fastq files were generated with bcl2fastq2 v2.19.0
Quality controlling and trimming was done using the Galaxy ToolShed v 1.0.2; FASTQ/A short-reads pre-processing tools
Aligned to the hg38 genome was done using HISAT2 (v 2.1.0+galaxy4)
Raw counts were generated using featureCounts (v 1.6.3+galaxy2)
Genome_build: hg38
Supplementary_files_format_and_content: Csv files containing raw counts for each replicate
|
| |
|
| Submission date |
Oct 20, 2020 |
| Last update date |
Sep 23, 2021 |
| Contact name |
Sofia Nordling |
| Organization name |
Uppsala University
|
| Street address |
Dag Hammarskölds väg 20
|
| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE159694 |
Retinoic Acid Promotes Endothelial Cell Cycle Early G1 State to Enable Human Hemogenic Endothelial Cell Specification |
|
| Relations |
| BioSample |
SAMN16485933 |
| SRA |
SRX9317705 |
