|
| Status |
Public on Sep 07, 2021 |
| Title |
RNAseq_WT26_p43 |
| Sample type |
SRA |
| |
|
| Source name |
WT_ESCs
|
| Organism |
Mus musculus |
| Characteristics |
cell line: WT26
genotype: wild type
cell type: mouse embryonic stem cells (ESCs)
|
| Growth protocol |
Feeder-independent ES cells were cultured on 10cm gelatin-coated plates with ES cell medium containing Serum and LIF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106) including the on-column DNAse step, ribosomal RNA was depleted using the RNARibo-Zero rRNA Removal Kit (Illumina, MRZH11124)
Libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, E7420S)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
GS275_ES_WT26p43_RNA_GATCAG
|
| Data processing |
Paired end RNA-seq reads were mapped to the mouse genome (mm10) using STAR (Dobin et al., 2013).
Genome_build: mm10
Supplementary_files_format_and_content: STAR output; reads per gene for each sample
|
| |
|
| Submission date |
Oct 23, 2020 |
| Last update date |
Sep 07, 2021 |
| Contact name |
Gunnar Schotta |
| E-mail(s) |
[email protected]
|
| Phone |
089218075422
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center
|
| Street address |
Grosshaderner Strasse 9
|
| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18480 |
| Series (2) |
| GSE159934 |
Morc3 silences endogenous retroviruses by enabling Daxx-mediated H3.3 incorporation [RNA-seq_wt/ko] |
| GSE159936 |
Morc3 silences endogenous retroviruses by enabling Daxx-mediated H3.3 incorporation |
|
| Relations |
| BioSample |
SAMN16523282 |
| SRA |
SRX9349869 |
