|
| Status |
Public on Apr 08, 2011 |
| Title |
Rescue Clone Minus Dox replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Rescue Clone Minus Dox replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Embryonic Stem Cell
transfection: Wdr5R4 -dox
|
| Growth protocol |
rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 103 U ml-1 of LIF (Chemicon) and doxycycline (1ug/ml)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Wdr5 KD through shRNA expression
|
| Data processing |
Data was VST normalized in R using LUMI and LIMMA packages
|
| |
|
| Submission date |
Dec 20, 2009 |
| Last update date |
Apr 08, 2011 |
| Contact name |
Jonathan Monk |
| E-mail(s) |
[email protected]
|
| Phone |
9716457785
|
| Organization name |
Mt. Sinai School of Medicine
|
| Department |
Gene and Cell Medicine
|
| Lab |
Lemischka
|
| Street address |
One Gustave L. Levy Place
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE19588 |
Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network (2) |
|
