|
| Status |
Public on Dec 01, 2020 |
| Title |
Patient 8 |
| Sample type |
RNA |
| |
|
| Source name |
Angiosarcoma tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Female
age: 53
Stage: Non-metastatic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using FFPE RNA/DNA Purification Plus Kit (Norgen) from five 10 μm tissue sections with adequate tumor available and analyzed using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). After excluding samples with suboptimal RNA integrity and content, the remaining samples were included in the nCounter analysis.
|
| Label |
biotin
|
| Label protocol |
100 ng total RNA was used as input into the hybridisation step and carried out according to the manufacturer's protocol
|
| |
|
| Hybridization protocol |
Hybridation was performed as per manufacturer's protocol
|
| Scan protocol |
Analysed in the nCounter Digital Analyser at 555 FOV
|
| Data processing |
Normalization was performed using the nSolver 4.0 Advanced Analysis software using default parameters
|
| |
|
| Submission date |
Nov 30, 2020 |
| Last update date |
Dec 01, 2020 |
| Contact name |
Jason Yongsheng Chan |
| E-mail(s) |
[email protected]
|
| Phone |
06564368000
|
| Organization name |
National Cancer Centre Singapore
|
| Street address |
11 Hospital Crescent
|
| City |
Singapore |
| ZIP/Postal code |
169690 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL27956 |
| Series (1) |
| GSE162370 |
Gene expression of human angiosarcoma and correlation with treatment outcomes |
|
