|
| Status |
Public on Aug 31, 2010 |
| Title |
CRI-G1-RR 03 |
| Sample type |
RNA |
| |
|
| Source name |
CRI-G1-RR 03
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: Rotenone resistant CRI-G1
|
| Biomaterial provider |
European Collection of Cell Cultures
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
Cells were grown in high glucose DMEM with Glutamax, supplemented with 10% FBS and antibotics; six different passages were collected in a paired fashion (one sample for each cell type) ~ 24 h after plating, pelleted and frozen at -80 C until tRNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
tRNA was extracted using Quiagen RNeasy kit with on column DNAse digerstion according to the manufacturer's instructions.
|
| Label |
BIOTIN
|
| Label protocol |
Biotinylated cRNA was prepared using Ambion MessageAmp II-Biotin Enhanced Kit according to the manufacturers's instructions, starting from 1 ug of tRNA/sample.
|
| |
|
| Hybridization protocol |
Affymetrix Rat 230 2.0 Array; GeneChip Expression Analysis Technical Manual 701021 Rev. 3
|
| Scan protocol |
GCS3000
|
| Description |
Rotenone resistant CRI-G1 cell line; biological sample #3
|
| Data processing |
GCOS 1.4
|
| |
|
| Submission date |
Jan 19, 2010 |
| Last update date |
Jun 21, 2010 |
| Contact name |
Natasha Chandiramani |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California San Francisco
|
| Department |
Pathology
|
| Lab |
Margeta
|
| Street address |
513 Parnassus Av
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE19948 |
Molecular basis for vulnerability to mitochondrial and oxidative stress in an insulinoma cell line |
|
