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Sample GSM49876 Query DataSets for GSM49876
Status Public on Dec 20, 2005
Title lateral motoneurons (LMN) 8, sham-operated control
Sample type RNA
 
Source name adult spinal cord, sham-operated control
Organism Mus musculus
Characteristics cell type: lateral motoneuron (LMN)
Extracted molecule total RNA
 
Description Three cell types, intermediolateral column motoneurons, medial motoneurons, and lateral motoneurons were isolated from a single adult spinal cord using laser capture microscopy. Four hundred captures were collected for each cell type. For a given cell type, RNA was extracted from the 400 captures using an Arcturus picopure kit. RNA was split in half and two targets were produced using a double amplification protocol. Each target was hybridized to Affymetrix chips and signals were normalized with R-pack. Inverse logs are provided. Five animals were used in these experiments, and all three cell types were collected from each animal. Thus, for each cell type, there are five biological replicates, and for each biological replicate there are two technical replicates. In all thirty chips were analyzed. Techinical replicates are indicated as Set 1 and Set 2. Animal numbers are indicated by Pair1 through Pair 5.
This sample is also known as sham mice. Set 2 - Pair 2 - LMN
 
Submission date Apr 28, 2005
Last update date Sep 22, 2009
Contact name Deborah Baro
E-mail(s) [email protected]
Organization name Georgia State University
Street address P.O. Box 4010
City Atlanta
State/province GA
ZIP/Postal code 30303
Country USA
 
Platform ID GPL81
Series (2)
GSE2595 Intermediolateral column, medial, and lateral motoneurons
GSE18179 Gene profiling of intermediolateral column, medial, and lateral motoneuron after spinal cord transection

Data table header descriptions
ID_REF Probeset ID
VALUE RMA Express calculated the Signal values

Data table
ID_REF VALUE
100001_at 36.216406
100002_at 732.826377
100003_at 24.398076
100004_at 155.765034
100005_at 177.570219
100006_at 48.445535
100007_at 226.233963
100009_r_at 520.010024
100010_at 54.291349
100011_at 80.584918
100012_at 313.193061
100013_at 94.636907
100014_at 225.169382
100015_at 126.895469
100016_at 44.580423
100017_at 45.922964
100018_at 167.80637
100019_at 284.881175
100020_at 253.002004
100021_at 28.701733

Total number of rows: 12488

Table truncated, full table size 250 Kbytes.




Supplementary file Size Download File type/resource
GSM49876.CEL.gz 2.9 Mb (ftp)(http) CEL

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