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Sample GSM49880 Query DataSets for GSM49880
Status Public on Dec 20, 2005
Title intermediolateral column motoneurons (IML) 12, sham-operated control
Sample type RNA
 
Source name adult spinal cord, sham-operated control
Organism Mus musculus
Characteristics cell type: intermediolateral column motoneuron (IML)
Extracted molecule total RNA
 
Description Three cell types, intermediolateral column motoneurons, medial motoneurons, and lateral motoneurons were isolated from a single adult spinal cord using laser capture microscopy. Four hundred captures were collected for each cell type. For a given cell type, RNA was extracted from the 400 captures using an Arcturus picopure kit. RNA was split in half and two targets were produced using a double amplification protocol. Each target was hybridized to Affymetrix chips and signals were normalized with R-pack. Inverse logs are provided. Five animals were used in these experiments, and all three cell types were collected from each animal. Thus, for each cell type, there are five biological replicates, and for each biological replicate there are two technical replicates. In all thirty chips were analyzed. Techinical replicates are indicated as Set 1 and Set 2. Animal numbers are indicated by Pair1 through Pair 5.
This sample is also known as sham mice. Set 2 - Pair 2 - IML
 
Submission date Apr 28, 2005
Last update date Sep 22, 2009
Contact name Deborah Baro
E-mail(s) [email protected]
Organization name Georgia State University
Street address P.O. Box 4010
City Atlanta
State/province GA
ZIP/Postal code 30303
Country USA
 
Platform ID GPL81
Series (2)
GSE2595 Intermediolateral column, medial, and lateral motoneurons
GSE18179 Gene profiling of intermediolateral column, medial, and lateral motoneuron after spinal cord transection

Data table header descriptions
ID_REF Probeset ID
VALUE RMA Express calculated the Signal values

Data table
ID_REF VALUE
100001_at 36.659436
100002_at 570.18186
100003_at 25.449028
100004_at 147.747659
100005_at 214.65135
100006_at 59.409993
100007_at 266.916427
100009_r_at 923.103746
100010_at 63.546862
100011_at 68.378086
100012_at 371.560187
100013_at 108.109388
100014_at 133.293247
100015_at 126.316792
100016_at 44.682424
100017_at 39.998692
100018_at 167.166787
100019_at 380.108823
100020_at 223.603963
100021_at 32.324531

Total number of rows: 12488

Table truncated, full table size 250 Kbytes.




Supplementary file Size Download File type/resource
GSM49880.CEL.gz 2.9 Mb (ftp)(http) CEL

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