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Sample GSM49893 Query DataSets for GSM49893
Status Public on Dec 20, 2005
Title lateral motoneurons (LMN) 25, sham-operated control
Sample type RNA
 
Source name adult spinal cord, sham-operated control
Organism Mus musculus
Characteristics cell type: lateral motoneuron (LMN)
Extracted molecule total RNA
 
Description Three cell types, intermediolateral column motoneurons, medial motoneurons, and lateral motoneurons were isolated from a single adult spinal cord using laser capture microscopy. Four hundred captures were collected for each cell type. For a given cell type, RNA was extracted from the 400 captures using an Arcturus picopure kit. RNA was split in half and two targets were produced using a double amplification protocol. Each target was hybridized to Affymetrix chips and signals were normalized with R-pack. Inverse logs are provided. Five animals were used in these experiments, and all three cell types were collected from each animal. Thus, for each cell type, there are five biological replicates, and for each biological replicate there are two technical replicates. In all thirty chips were analyzed. Techinical replicates are indicated as Set 1 and Set 2. Animal numbers are indicated by Pair1 through Pair 5.
This sample is also known as sham mice. Set 1 - Pair 5 - LMN
 
Submission date Apr 28, 2005
Last update date Sep 22, 2009
Contact name Deborah Baro
E-mail(s) [email protected]
Organization name Georgia State University
Street address P.O. Box 4010
City Atlanta
State/province GA
ZIP/Postal code 30303
Country USA
 
Platform ID GPL81
Series (2)
GSE2595 Intermediolateral column, medial, and lateral motoneurons
GSE18179 Gene profiling of intermediolateral column, medial, and lateral motoneuron after spinal cord transection

Data table header descriptions
ID_REF Probeset ID
VALUE RMA Express calculated the Signal values

Data table
ID_REF VALUE
100001_at 47.911458
100002_at 459.316457
100003_at 25.288908
100004_at 132.554208
100005_at 213.597089
100006_at 41.442618
100007_at 267.49041
100009_r_at 552.610141
100010_at 64.834567
100011_at 77.172832
100012_at 336.673694
100013_at 83.409425
100014_at 156.71546
100015_at 135.893341
100016_at 44.238595
100017_at 50.810742
100018_at 207.620707
100019_at 231.009023
100020_at 263.35834
100021_at 34.01431

Total number of rows: 12488

Table truncated, full table size 250 Kbytes.




Supplementary file Size Download File type/resource
GSM49893.CEL.gz 2.8 Mb (ftp)(http) CEL

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