NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM49895 Query DataSets for GSM49895
Status Public on Dec 20, 2005
Title medial motoneurons (MMN) 27, sham-operated control
Sample type RNA
 
Source name adult spinal cord, sham-operated control
Organism Mus musculus
Characteristics cell type: medial motoneuron (MMN)
Extracted molecule total RNA
 
Description Three cell types, intermediolateral column motoneurons, medial motoneurons, and lateral motoneurons were isolated from a single adult spinal cord using laser capture microscopy. Four hundred captures were collected for each cell type. For a given cell type, RNA was extracted from the 400 captures using an Arcturus picopure kit. RNA was split in half and two targets were produced using a double amplification protocol. Each target was hybridized to Affymetrix chips and signals were normalized with R-pack. Inverse logs are provided. Five animals were used in these experiments, and all three cell types were collected from each animal. Thus, for each cell type, there are five biological replicates, and for each biological replicate there are two technical replicates. In all thirty chips were analyzed. Techinical replicates are indicated as Set 1 and Set 2. Animal numbers are indicated by Pair1 through Pair 5.
This sample is also known as sham mice. Set 1 - Pair 5 - MMN
 
Submission date Apr 28, 2005
Last update date Sep 22, 2009
Contact name Deborah Baro
E-mail(s) [email protected]
Organization name Georgia State University
Street address P.O. Box 4010
City Atlanta
State/province GA
ZIP/Postal code 30303
Country USA
 
Platform ID GPL81
Series (2)
GSE2595 Intermediolateral column, medial, and lateral motoneurons
GSE18179 Gene profiling of intermediolateral column, medial, and lateral motoneuron after spinal cord transection

Data table header descriptions
ID_REF
VALUE RMA Express calculated the Signal values

Data table
ID_REF VALUE
100001_at 48.058604
100002_at 699.98949
100003_at 27.097457
100004_at 135.957168
100005_at 196.367166
100006_at 50.934116
100007_at 238.185871
100009_r_at 824.085345
100010_at 60.31823
100011_at 84.908696
100012_at 315.988415
100013_at 71.818954
100014_at 181.388649
100015_at 139.705842
100016_at 45.957492
100017_at 53.771844
100018_at 200.891768
100019_at 307.843352
100020_at 209.00218
100021_at 27.750477

Total number of rows: 12488

Table truncated, full table size 250 Kbytes.




Supplementary file Size Download File type/resource
GSM49895.CEL.gz 2.7 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap