|
| Status |
Public on Apr 09, 2010 |
| Title |
Pou4f1+/+ cells, transduced with MSCV-Pou4f1 virus_WT-POU_82 |
| Sample type |
RNA |
| |
|
| Source name |
Pou4f1+/+ cells, transduced with MSCV-Pou4f1 virus
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Pou4f1+/+
agent: MSCV-Pou4f1 virus
tissue type: fetal liver
|
| Treatment protocol |
Cells were transduced two consecutive days with MSCV-based retroviruses and sorted on GFP 48 hours later.
|
| Growth protocol |
Fetal liver cells were removed, subjected to red cell lysis, and grown for 48h in RPMI 20% FBS, 1% L-Glutamine supplemented with 10 ng/mL interleukin-6, 100 ng/mL SCF, 6 ng/mL IL3, 50 ng/mL FLT3 ligand and 10 ng/mL TPO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol LS (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
RNA was linear amplified
|
| |
|
| Hybridization protocol |
According to Affymetrix protocol
|
| Scan protocol |
According to Affymetrix protocol
|
| Description |
WT-POU 74-010909-0900818
|
| Data processing |
Raw probe intensities were background-corrected, quantile-normalized, and summarized using the R function rma.
|
| |
|
| Submission date |
Jan 22, 2010 |
| Last update date |
Apr 09, 2010 |
| Contact name |
Patrick Cahan |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
ICE and Biomedical Engineering
|
| Lab |
Cahan Lab
|
| Street address |
733 North Broadway, MRB 653
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE19997 |
POU4F1 is associated with t(8;21) AML and contributes directly to its unique transcriptional signature |
|
