|
| Status |
Public on Sep 27, 2021 |
| Title |
Car_CD34p_1 |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ tumor cells from DMBA tumor
|
| Organism |
Mus musculus |
| Characteristics |
tumor type: Carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
|
| Label |
biotin
|
| Label protocol |
Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
|
| |
|
| Hybridization protocol |
Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
|
| Scan protocol |
Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
|
| Description |
Gene expression data from Nr2f2 carcinoma
|
| Data processing |
To normalize the data, the Robust Multi-array Average expression (RMA) method from the affy package was used.
|
| |
|
| Submission date |
Jan 11, 2021 |
| Last update date |
Sep 27, 2021 |
| Contact name |
Yura Song |
| E-mail(s) |
[email protected]
|
| Organization name |
Université Libre de Bruxelles
|
| Lab |
Laboratory of Stem Cells and Cancer
|
| Street address |
808, route de Lennik
|
| City |
Bruxelles |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE164597 |
NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation II |
| GSE164605 |
NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation |
|
