|
| Status |
Public on Apr 23, 2010 |
| Title |
Reh_mock1 |
| Sample type |
RNA |
| |
|
| Source name |
Reh, control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Reh
transfection: control shRNA
malignancy: non-Hodgkin lymphoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini-Kit from QIAGEN according to the manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
Labeling was done according to the manufacturer's recommendations (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was done according to the manufacturer's recommendations (Affymetrix).
|
| Scan protocol |
Scanning was done according to the manufacturer's recommendations (Affymetrix). Scanner Model was GeneChip® Scanner 3000 7G. GeneChip® Command Console® 2.0 was used as software.
|
| Description |
Biological replicate 1 of 2.
|
| Data processing |
Raw data were background corrected with RMA and normalized using quantile normalization. All processing was done in R/Bioconductor.
|
| |
|
| Submission date |
Jan 29, 2010 |
| Last update date |
Apr 23, 2010 |
| Contact name |
Karl Koechert |
| E-mail(s) |
[email protected]
|
| Organization name |
Max-Delbrueck-Centre for Molecular Medicine
|
| Street address |
Robert-Roessle-Str. 10
|
| City |
Berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE20115 |
Expression analysis of Reh cells after transfection with shRNA targeting CBFA2T3 and/or constitutively active IKKβ(EE) |
|
