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| Status |
Public on Jun 15, 2022 |
| Title |
C1: un-injected at 1hpf |
| Sample type |
SRA |
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| Source name |
zebrafish embryos
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| Organism |
Danio rerio |
| Characteristics |
strain: TU
developmental stage: 1hpf
tissue: embryo
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| Treatment protocol |
In the 1-cell stage of zebrafish embryos, 8ng/μL pEGFP-N1 was injected, and the embryos of the injected group and the non-injected group were collected when the embryos developed to 1hpf, 6hpf and 12hpf. Add 1mL TRIzol (Invitrogen, USA), store at -80℃
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen).
RNA library using standard Ion proton RNA-Seq Kit v2.0 protocol
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
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| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
Clean Reads mapped to GRCz10 genome using Mapsplice v2.1.8 with parameters -s 22 -p 15 --ins 6 --del 6 --non-canonical --bam -o
count counting
Genome_build: GRCz10
Supplementary_files_format_and_content: TXT file contain counts for each sample
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| Submission date |
Jan 25, 2021 |
| Last update date |
Jun 15, 2022 |
| Contact name |
Nan Li |
| E-mail(s) |
[email protected]
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| Phone |
15005162573
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| Organization name |
Qingshun Zhao
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| Street address |
No.12, Xuefu Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210000 |
| Country |
China |
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| Platform ID |
GPL24059 |
| Series (1) |
| GSE165422 |
Next Generation Sequencing Facilitates Quantitative Analysis of different developmental stage pEGFP-N1 injected zebrafish embryos and untreated group transcriptomes |
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| Relations |
| BioSample |
SAMN17527727 |
| SRA |
SRX9931395 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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