|
| Status |
Public on Jan 30, 2021 |
| Title |
Hela cells, SRSF10-overexpression, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Hela cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
genotype: SRSF10-overexpression
treatment: untreated
|
| Treatment protocol |
untreated
|
| Growth protocol |
The Hela cells of control and SRSF10-overexpression were digested with trypsin,and then divided into 3 6cm-dishes respectively. Cell were harvested after 24h culturing in DMEM medium plus 10% FBS and 1% Penicillin-Streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The medium were removed and cells were washed with ice PBS buffer. RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-T7 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Homo sapiens whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata
RSEM software was used to calculate gene expression level (FPKM).
Genome_build: GRCh38.p12, GCF_000001405.38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample. Gene IDs are NCBI Gene IDs.
|
| |
|
| Submission date |
Jan 29, 2021 |
| Last update date |
Jan 31, 2021 |
| Contact name |
Chenhui Wang |
| E-mail(s) |
[email protected]
|
| Phone |
+8615927460122
|
| Organization name |
Huazhong University of Science and Technology
|
| Street address |
1037 Luoyu Rd
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430074 |
| Country |
China |
| |
|
| Platform ID |
GPL29480 |
| Series (1) |
| GSE165781 |
Identification of LncRNA TRAF3IP2-AS1 as a key regulator of IL-17 signaling through the SRSF10-IRF1-Act1 axis in autoimmune diseases |
|
| Relations |
| BioSample |
SAMN17676540 |
| SRA |
SRX9975605 |
