|
| Status |
Public on Feb 01, 2011 |
| Title |
CLL 379-01 LMP-420 |
| Sample type |
RNA |
| |
|
| Source name |
CLL cells
|
| Organism |
Homo sapiens |
| Characteristics |
agent: LMP-420
selection protocol: negatively selected cells
|
| Treatment protocol |
Incubation with 2 uM LMP-420 or media alone
|
| Growth protocol |
24 hour incubation in Hybridoma SFM media with 10% fetal bovine serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiashredder and RNeasy Mini Columns
|
| Label |
biotin
|
| Label protocol |
According to standard Affymetrix protocol. Affymetrix 1-step IVT
|
| |
|
| Hybridization protocol |
According to standard Affymetrix protocol.
|
| Scan protocol |
According to standard Affymetrix protocol.
|
| Description |
Treated cells
|
| Data processing |
R/Bioconductor; RMA normalization
|
| |
|
| Submission date |
Feb 05, 2010 |
| Last update date |
Feb 01, 2011 |
| Contact name |
Daphne R Friedman |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University Medical Center
|
| Street address |
101 Science Drive, Room 2121, CIEMAS Bldg
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE20211 |
LMP-420: a novel purine nucleoside analogue with potent cytotoxic effects for chronic lymphocytic leukemia cells |
|
