|
| Status |
Public on Feb 18, 2021 |
| Title |
MDAMB-RNA1: YAPi_TAZi_MDAMB231_2 |
| Sample type |
SRA |
| |
|
| Source name |
Breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231 cells
transformation status: --
|
| Treatment protocol |
1-0.4 μM Tamoxifen (Sigma, H7904) + 2-4 μM AZD0530 (Selleck Chemicals, S1006) were used to induce the transformation.
|
| Growth protocol |
MCF-10A-ER-Src cells (Iliopoulos et al., 2009; Iliopoulos et al., 2010) were grown in DMEM/F12 without phenol red (Thermo Fisher Scientific, 11039-047) + 5% charcoal stripped FBS (Sigma, F6765) + 1% pen/strep (Thermo Fisher Scientific, 15140122)+20 ng/ml EGF (Peprotech, AF-100-15) + 0.5 μg/ml Hydrocortisone (Sigma, H-0888) + 0.1 μg/ml cholera toxin (Sigma, C-8052) + 10 μg/ml insulin (Sigma, 10516).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared used mRNeasy Mini Kit (Qiagen, No. 217004).
RNA-seq libraries were generated using TruSeq Ribo Profile Mammalian Kit (Illumina, RPHMR12126).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
base-calling performed by Illumina Hiseq 2000
Trim adapter
Align reads using STAR
Estimate genecounts using RSEM
Genome_build: hg38
|
| |
|
| Submission date |
Feb 17, 2021 |
| Last update date |
Feb 18, 2021 |
| Contact name |
Kevin Struhl |
| E-mail(s) |
[email protected]
|
| Phone |
617-432-2104
|
| Fax |
617-432-2529
|
| Organization name |
Harvard Medical School
|
| Department |
Biological Chemistry and Molecular Pharamcology
|
| Lab |
Kevin Struhl
|
| Street address |
240 Longwood Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE166942 |
YAP and TAZ are transcriptional co-activators of AP-1 proteins and STAT3 during breast cellular transformation (RNA-seq) |
| GSE166943 |
YAP and TAZ are transcriptional co-activators of AP-1 proteins and STAT3 during breast cellular transformation |
|
| Relations |
| BioSample |
SAMN17951150 |
| SRA |
SRX10119310 |
