|
| Status |
Public on Feb 28, 2010 |
| Title |
Peripheral blood RNA from sample 25 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from peripheral blood RNA.
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PAXgene Blood RNA isolation kit protocol (Qiagen, UK, 762174)
|
| Label |
Cy3
|
| Label protocol |
2.5 ml of peripheral blood was collected into a PAXgene tube (Becton Dickinson, UK, 762165). PAXgene vials were chosen to prevent density gradient centrifugation, immortalization or in vitro cell culture artifacts changing mRNA profiles. PAXgene tubes were mixed gently and incubated at room temperature for two hours. After collection, tubes were frozen at -20 degrees Celsius for at least 24 hours followed by storage at -80 degrees Celsius. RNA was isolated using the PAXgene Blood RNA isolation kit (Qiagen, UK, 762174). RNA was quantified using the Nanodrop (Nanodrop Technologies, USA). Total RNA integrity was analyzed using an Agilent Bioanalyzer (Agilent Technologies, USA).
|
| |
|
| Hybridization protocol |
Complementary RNA was hybridized to Illumina HumanRef H8-v2.0 Whole Genome BeadChips
|
| Scan protocol |
Illumina BeadArray Reader, data was handled through Illumina BeadStudio Gene Expression module v3
|
| Description |
Gene expression from whole peripheral blood from unrelated Dutch or UK individuals.
|
| Data processing |
Quantile-quantile normalized log2 transformed expression intensity. Subsequently, per sample the intensities have been centered to zero and subsequently scaled such that each individual has a standard deviation of 1 in probe
|
| |
|
| Submission date |
Feb 16, 2010 |
| Last update date |
Feb 18, 2010 |
| Contact name |
Lude Franke |
| E-mail(s) |
[email protected]
|
| Phone |
+316 41549962
|
| Organization name |
University Medical Centre Groningen
|
| Department |
Genetics
|
| Lab |
Genetics
|
| Street address |
P.O. Box 30001
|
| City |
Groningen |
| State/province |
Groningen |
| ZIP/Postal code |
9700 RR |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE20332 |
Primary human leukocyte RNA expression of unrelated Dutch and UK individuals |
|
