|
| Status |
Public on Feb 19, 2010 |
| Title |
wild type biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
R. sphaeroides 2.4.1
|
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Characteristics |
genotype: wild type
|
| Treatment protocol |
Total RNA was extracted directly from the culture.
|
| Growth protocol |
Strains were sparged anaerobicallyin the dark with 95%N2-5%CO2 in SIS medium supplemented with 0.1% yeast extract in the presence of DMSO (0.5% vol/vol), as electron acceptor. When the optical density at 600 nm (OD600) reached approximately 0.4 RNA was isolated from a 50-ml aliquot of the culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by a modified hot-phenol method described in J. Biol. Chem. 279:9146-9155, 2004
|
| Label |
biotin
|
| Label protocol |
cDNA synthesized from total RNA was fragmented and labeled using the Enzo BioArray terminal labeling kit
|
| |
|
| Hybridization protocol |
Target hybridization, staining, and scanning were performed according to the instructions designed for the Pseudomonas aeruginosa genome array by Affymetrix
|
| Scan protocol |
Agilent GeneArray scanner under the control of the Affymetrix GCOS
|
| Description |
Gene expression data from anaerobic dark/DMSO condition
|
| Data processing |
.CEL file converted to MAS5 format and analyzed using Affymetrix MAS5 default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 16, 2010 |
| Last update date |
Feb 18, 2010 |
| Contact name |
Jung Hyeob Roh |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
713-500-5437
|
| Organization name |
The University of Texas
|
| Department |
Microbiology & Molecular Genetics
|
| Street address |
6431 Fannin St.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL162 |
| Series (1) |
| GSE20337 |
Role of the global transcriptional regulator PrrA in R. sphaeroides 2.4.1 |
|
