|
| Status |
Public on Nov 11, 2022 |
| Title |
HEK293-4A cells rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic kidney cells
|
| Organism |
Homo sapiens |
| Characteristics |
strain: HEK293
treatment: untreated
genotype: Myc-tagged 4A mutant
|
| Treatment protocol |
20 ng/ml EGF for 60 minutes
|
| Growth protocol |
HEK293-WT and HEK293-4A cells were seeded on 100 mm dish and cultured for 18 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN).
RNA libraries were prepared using 100 ng of total RNA with an Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
The sequencing reads were aligned to hg19_AmpliSeq_Transcriptome_ERCC_v1 using Torrent Mapping Alignment Program (TMAP).
QC metrics and normalized read counts per gene were obtained using AmpliSeqRNA plug-in v5.2.0.3, a Torrent Suite Software v5.2.2 (Thermo Fisher Scientific).
Genome_build: hg19_AmpliSeq_Transcriptome_ERCC_v1
Supplementary_files_format_and_content: Tab-delimited text files include RPM values for each Sample.
|
| |
|
| Submission date |
Feb 22, 2021 |
| Last update date |
Nov 11, 2022 |
| Contact name |
Mutsuhiro Takekawa |
| Organization name |
The University of Tokyo
|
| Department |
Institute of Medical Science
|
| Lab |
Division of Cell Signaling and Molecular Medicine
|
| Street address |
4-6-1
|
| City |
Shirokanedai |
| State/province |
Minato-ku, Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE167233 |
A novel ERK substrate regulates wide varieries of IEGs |
|
| Relations |
| BioSample |
SAMN18026328 |
| SRA |
SRX10149700 |
