cultured cell type: VND6 culture cell time point: 12h
Treatment protocol
cultured cells were treated with 2 μM estrogen for 0h and 12h
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted with TRIzol (Invitrogen) and purified with Rneasy Micro Kit (QIAGEN)
Label
biotin
Label protocol
Biotinylated cRNA was prepared from total RNA with GeneChip One-Cycle Target Labeling and Control Reagents (Arab-0018 to Arab-0054) or GeneChip 3' IVT Expression Kit (Arab-113-2 to Arab-124) (Affymetrix).
Hybridization protocol
Hybridization, washing, staining procedures were conducted with Fluidics Station 450 as described in the GeneChip Expression Analysis Technical Manual (Affymetrix).
Scan protocol
Scanning procedure was conducted with GeneChip Scanner 3000 7G as described in the GeneChip Expression Analysis Technical Manual (Affymetrix).
Description
Estrogen-inducible VND6 transformed cells with 12h estrogen treatment
